The National Underground Railroad Freedom Center is a museum in downtown Cincinnati, Ohio, based on the history of the Underground Railroad. Opened in 2004, the center also pays tribute to all efforts to "abolish human enslavement and secure freedom for all people".

It is one of a new group of "museums of conscience" in the United States, along with the Museum of Tolerance, the United States Holocaust Memorial Museum and the National Civil Rights Museum. The center offers insight into the struggle for freedom in the past, in the present, and for the future, as it attempts to challenge visitors to contemplate the meaning of freedom in their own lives. Its location recognizes the significant role of Cincinnati in the history of the Underground Railroad, as thousands of slaves escaped to freedom by crossing the Ohio River from the southern slave states. Many found refuge in the city, some staying there temporarily before heading north to gain freedom in Canada.

The Cincinnati Zoo & Botanical Garden is the sixth oldest zoo in the United States, founded in 1873 and officially opening in 1875. It is located in the Avondale neighborhood of Cincinnati, Ohio. It originally began with 64.5 acres (26.1 ha) in the middle of the city, but has spread into the neighboring blocks and several reserves in Cincinnati's outer suburbs. It was appointed as a National Historic Landmark in 1987.

The zoo houses over 500 species, 1,800 animals and 3,000 plant species. In addition, the zoo also has conducted several breeding programs in its history, and was the first to successfully breed California sea lions. In 1986, the Lindner Center for Conservation and Research of Endangered Wildlife (CREW) was created to further the zoo's goal of conservation. The zoo is known for being the home of Martha, the last living passenger pigeon, and of Incas, the last living Carolina parakeet.

A 2014 ranking of the nations's best zoos by USA Today based on data provided by the Association of Zoos and Aquariums lists the Cincinnati Zoo among the best in the country. A 2019 reader's choice ranking of the nation's best zoos by USA Today named the Cincinnati Zoo the top zoo in North America.

The Cincinnati History Museum is an urban history museum in Cincinnati, Ohio, United States. It opened in 1990 at the Cincinnati Museum Center at Union Terminal.

The museum features the recreated Cincinnati Public Landing. Explore a recreation of the bustling Public Landing from the late 1850s and climb aboard the Queen of the West, a replica side-wheel steamboat. Take an aerial view of Cincinnati from the early 1900s through 1940s in Cincinnati in Motion, a 1/64-scale replica of the city complete with the nation’s largest S-scale train model. Cincinnati In Motion is a scale model representation of Downtown Cincinnati in the 1940s featuring working streetcars.

The Cincinnati Art Museum is an art museum in the Eden Park neighborhood of Cincinnati, Ohio. Founded in 1881, it was the first purpose-built art museum west of the Alleghenies, and is one of the oldest in the United States. Its collection of over 67,000 works spanning 6,000 years of human history make it one of the most comprehensive collections in the Midwest.

Krohn Conservatory opened in 1933 and is located in Eden Park. The land in the park used to belong to Nicholas Longworth and he called it his Garden of Eden.

The conservatory was completed in 1933, replacing smaller greenhouses that had stood in Eden Park since 1894. Originally known only as the Eden Park Greenhouse, in 1937 it was renamed in honor of Irwin M. Krohn, who served as Board of Park Commissioner from 1912 to 1948. The architect firm Rapp & Meacham designed the conservatory in the Art Deco style, in the form of a Gothic arch.